Mass Spectrometric Determination of the Effect of Surface Deactivation on Membranes Used for In-Situ Sampling of Cerebrospinal Fluid (CSF) [Elektronisk resurs]
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Undin, Torgny, 1975- (författare)
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Dahlin, Andreas P., 1976- (författare)
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Bergquist, Jonas (författare)
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Bergström Lind, Sara, 1977- (författare)
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Uppsala universitet Teknisk-naturvetenskapliga vetenskapsområdet (utgivare)
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Uppsala universitet Teknisk-naturvetenskapliga vetenskapsområdet (utgivare)
- Publicerad: 2018
- Engelska.
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Ingår i: ; 5:2
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Sammanfattning
Ämnesord
Stäng
- In this paper, a strategy for structured monitoring of surface modifications to control protein adsorption to membrane structures is presented. The already established on-surface enzymatic digestion (oSED) method combined with nano-liquid chromatography and tandem mass spectrometry (LC-MS/MS) analysis was employed for the analysis of proteins in ventricular cerebrospinal fluid (vCSF) from neurointensive care patients. Protein adsorption was studied by in-situ sampling in a temporally resolved manner on both immobilized native and Pluronic-deactivated membranes. Deactivation was significantly reducing the protein adsorption but it also induced novel selective properties of the surface. The proposed versatile strategy will facilitate protein-biomaterial, protein-polymer, protein-protein interaction studies in the future.
Ämnesord
- Natural Sciences (hsv)
- Chemical Sciences (hsv)
- Analytical Chemistry (hsv)
- Naturvetenskap (hsv)
- Kemi (hsv)
- Analytisk kemi (hsv)
- Kemi med inriktning mot analytisk kemi (uu)
- Chemistry with specialization in Analytical Chemistry (uu)
- Chemistry with specialization in Surface Biotechnology (uu)
- Kemi med inriktning mot ytbioteknik (uu)
Indexterm och SAB-rubrik
- Protein Adsorption
- Mass Spectrometry
- oSED
- Surface modification
- Coating
- Poloxamer 407
- Pluronic F127
- Trypsin
- Digestion
- Shotgun
- Bottom-up
- peptides
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