Mass Spectrometric Determination of the Effect of Surface Deactivation on Membranes Used for In-Situ Sampling of Cerebrospinal Fluid (CSF) [Elektronisk resurs]

• In this paper, a strategy for structured monitoring of surface modifications to control protein adsorption to membrane structures is presented. The already established on-surface enzymatic digestion (oSED) method combined with nano-liquid chromatography and tandem mass spectrometry (LC-MS/MS) analysis was employed for the analysis of proteins in ventricular cerebrospinal fluid (vCSF) from neurointensive care patients. Protein adsorption was studied by in-situ sampling in a temporally resolved manner on both immobilized native and Pluronic-deactivated membranes. Deactivation was significantly reducing the protein adsorption but it also induced novel selective properties of the surface. The proposed versatile strategy will facilitate protein-biomaterial, protein-polymer, protein-protein interaction studies in the future. 

Ämnesord

Natural Sciences  (hsv)

Chemical Sciences  (hsv)

Analytical Chemistry  (hsv)

Naturvetenskap  (hsv)

Kemi  (hsv)

Analytisk kemi  (hsv)

Kemi med inriktning mot analytisk kemi  (uu)

Chemistry with specialization in Analytical Chemistry  (uu)

Chemistry with specialization in Surface Biotechnology  (uu)

Kemi med inriktning mot ytbioteknik  (uu)

Indexterm och SAB-rubrik

Protein Adsorption

Mass Spectrometry

oSED

Surface modification

Coating

Poloxamer 407

Pluronic F127

Trypsin

Digestion

Shotgun

Bottom-up

peptides