The PCR Revolution basic technologies and applications / edited by Stephen A. Bustin.

• BASIC TECHNOLOGIES -- Real-time polymerase chain reaction / Mickey Williams -- Thermostable enzymes used in polymerase chain reaction / Sudip K. Rakshit -- Inventing molecular beacons / Fred Russell Kramer, Salvatore A.E. Marras, and Sanjay Tyagi -- Rapid polymerase chain reaction and melting analysis / Carl T. Wittwer, Randy P. Rasmussen, and Kirk M. Ririe -- Polymerase chain reaction and fluorescence chemistries : deoxyribonucleic acid incarnate / Ben Sowers -- Analysis of microribonucleic acid expression by quantitative real-time polymerase chain reaction / Vladimir Benes ... [et al.] -- Miniaturized polymerase chain reaction for quantitative clinical diagnostics / Melissa Mariani, Lin Chen, and Philip J. Day -- The road from qualitative to quantitative assay : what is next? / Michael W. Pfaffl -- Taking control of the polymerase chain reaction / Tania Nolan, Tanya Novak, and Jim Huggett -- APPLICATIONS -- Polymerase chain reaction-based methods for the detection of solid tumor cancer cells for clinical diagnostic and prognostic assays / Susan A. Burchill -- Polymerase chain reaction and infectious diseases / Jim Huggett -- Polymerase chain reaction and respiratory viruses / Ian M. Mackay -- Polymerase chain reaction and severe acute respiratory syndrome / Weijun Chen and Yang Huanming -- The MMR vaccine, measles virus, and autism : a cautionary tale / Stephen A. Bustin -- Noninvasive prenatal diagnosis using cell-free fetal nucleic acids in maternal plasma / Y.M. Dennis Lo -- Polymerase chain reaction-based analyses of nucleic acids from archival material / Ulrich Lehmann -- Microarrays and quantitative real-time reverse transcriptase-polymerase chain reaction / Elisa Wurmbach -- Polymerase chain reaction in the detection of genetic variation / Pui-Yan Kwok -- Polymerase chain reaction : a blessing and a curse for ancient deoxyribonucleic acid research / Michael Hofreiter and Holger Römpler. 

• The invention of the polymerase chain reaction (PCR) won the Nobel Prize in Chemistry in 1994 and remains one of the most important scientific discoveries of the twentieth century. More than 50,000 researchers in the United States use PCR replication technology, and yet a book has not been published on the subject in more than ten years. In this book, Dr. Stephen A. Bustin, a world-renowned PCR expert, examines in detail the latest innovations and the overall impact of PCR on many areas of molecular research. The book contains personal reflections, opinions, and comments by leading authorities on the many applications of the PCR and how this technology has revolutionized their respective areas of interest. This book conveys the ways in which PCR has overcome many obstacles in life science and clinical research and also charts the PCR’s development from time-consuming, low throughput, non-quantitative procedure to today’s rapid, high throughput, quantitative super method. 

Ämnesord

Diagnostic Techniques and Procedures.  (MeSH)

Polymerase Chain Reaction  -- methods. (MeSH)

Nucleic Acids  -- analysis. (MeSH)

Genre

Electronic books.  (LCSH)

Klassifikation

572.43 (DDC)

Ue.043 (kssb/8 (machine generated))