Super-resolving microscopy reveals the localizations and movement dynamics of stressosome proteins in Listeria monocytogenes [Elektronisk resurs]
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Tran, Buu Minh (författare)
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Linnik, Dmitrii Sergeevich (författare)
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Punter, Christiaan Michiel (författare)
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Śmigiel, Wojciech Mikołaj (författare)
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Mantovanelli, Luca (författare)
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Iyer, Aditya (författare)
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O’Byrne, Conor (författare)
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Abee, Tjakko (författare)
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Johansson, Jörgen (författare)
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Poolman, Bert (författare)
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Umeå universitet Medicinska fakulteten (utgivare)
- Publicerad: Nature Publishing Group, 2023
- Engelska.
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Ingår i: Communications Biology. ; 6:1
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- Relaterad länk:
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http://www.umu.se/ (Värdpublikation)
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- The human pathogen Listeria monocytogenes can cope with severe environmental challenges, for which the high molecular weight stressosome complex acts as the sensing hub in a complicated signal transduction pathway. Here, we show the dynamics and functional roles of the stressosome protein RsbR1 and its paralogue, the blue-light receptor RsbL, using photo-activated localization microscopy combined with single-particle tracking and single-molecule displacement mapping and supported by physiological studies. In live cells, RsbR1 is present in multiple states: in protomers with RsbS, large clusters of stressosome complexes, and in connection with the plasma membrane via Prli42. RsbL diffuses freely in the cytoplasm but forms clusters upon exposure to light. The clustering of RsbL is independent of the presence of Prli42. Our work provides a comprehensive view of the spatial organization and intracellular dynamics of the stressosome proteins in L. monocytogenes, which paves the way towards uncovering the stress-sensing mechanism of this signal transduction pathway.
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- Natural Sciences (hsv)
- Biological Sciences (hsv)
- Biochemistry and Molecular Biology (hsv)
- Naturvetenskap (hsv)
- Biologiska vetenskaper (hsv)
- Biokemi och molekylärbiologi (hsv)
- Medical and Health Sciences (hsv)
- Basic Medicine (hsv)
- Microbiology in the medical area (hsv)
- Medicin och hälsovetenskap (hsv)
- Medicinska och farmaceutiska grundvetenskaper (hsv)
- Mikrobiologi inom det medicinska området (hsv)
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Communications Biology