Novel assay to improve therapeutic drug monitoring of thiopurines in inflammatory bowel disease [Elektronisk resurs]
-
Vikingsson, Svante (författare)
-
Andersson, David (författare)
-
Almer, Sven (författare)
-
Peterson, Curt (författare)
-
Hindorf, Ulf (författare)
-
- Linköpings universitet Institutionen för medicin och hälsa (utgivare)
-
-
Alternativt namn: IMH
-
Alternativt namn: Engelska: Linköping Universty. Department of Medical and Health Sciences
-
Alternativt namn: Linköping Universty. Department of Medicine and Health Sciences
-
Se även: Linköpings universitet. Institutionen för hälsa och samhälle
-
Se även: Linköpings universitet. Institutionen för medicin och vård
-
Linköpings universitet Hälsouniversitetet (utgivare)
-
- Linköpings universitet Institutionen för klinisk och experimentell medicin (utgivare)
-
-
Alternativt namn: IKE
-
Alternativt namn: Linköping University. Department of Clinical and Experimental Medicine
-
Alternativt namn: Linköping University. Faculty of Health Sciences. Department of Clinical and Experimental Medicine
-
Östergötlands Läns Landsting Hjärt- och Medicincentrum (utgivare)
-
Östergötlands Läns Landsting Centrum för kirurgi, ortopedi och cancervård (utgivare)
- Elsevier 2014
- Engelska.
-
Ingår i: Journal of Crohn's & Colitis. - 1873-9946. ; 8:12, 1702-1709
-
Läs hela texten
-
Läs hela texten
-
Läs hela texten
Sammanfattning
Ämnesord
Stäng
- Background and aims: The thiopurines are widely used in the treatment of inflammatory bowel disease, but are limited by poor dose effect relationship. The objective was to assess the ability of a novel assay, determining the mono-, di-, and triphosphates, of thioguanine as well as methylthioinosine as individual metabolites in erythrocytes, to predict clinical outcome compared to a routine assay, determining metabolites as sums. Methods: Samples from 79 patients with Crohns disease or ulcerative colitis treated with azathioprine or mercaptopurine were analysed by both assays. Clinical status was determined by the Harvey-Bradshaw and Walmsley indices. The genotypes of thiopurine methyltransferase (TPMT) and inosine triphosphatase were determined. Results: TPMT wild-type patients with thioguanine nucleotide (TGN) levels below the cut-off level were more likely to have active disease when TGN was measured by the novel assay (p = 0.02), and when thioguanosine triphosphate (TGTP) was measured separately (p = 0.01). When TGN was measured by the routine assay the correlation was not evident (p = 0.12). Neither TGN levels nor TGTP correlated to disease activity in TPMT deficient patients. Patients with methyl thioinosine nucleotide (meTIN) levels above 1500 pmol/8 x 10less than^greater than8 RBCs were more likely to have active disease (p = 0.07). We observed good correlations between the mono-, di-, and triphosphates and their respective sums (R-2 greater than 0.88). Conclusions: The novel TGN assay was better in predicting clinical outcome compared to the routine assay, while determination of TGTP had no clinical advantage and TGTP ratio was not correlated to disease activity. (C) 2014 European Crohns and Colitis Organisation. Published by Elsevier B.V. All rights reserved.
Ämnesord
- Medical and Health Sciences (hsv)
- Clinical Medicine (hsv)
- Medicin och hälsovetenskap (hsv)
- Klinisk medicin (hsv)
Indexterm och SAB-rubrik
- Purines; HPLC; Inflammatory bowel diseases; Individualized medicine; Thiopurine; Azathioprine
Inställningar
Hjälp
Beståndsinformation saknas