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Resin Microscopy and On-Section Immunocytochemistry
Geoffrey R. Newman (Författare), Jan A. Hobot (Författare, Författare, http://id.loc.gov/vocabulary/relators/aut)
DOI 10.1007/978-3-642-56930-2, ISBN 3642569307
2nd ed. 2001.
utgivning
Berlin, Heidelberg : Springer Berlin Heidelberg; Imprint: Springer, 20011 online resource (XIX, 273 p.)
Seriemedlemskap
Springer Lab Manuals · Springer Lab Manualsby Geoffrey R. Newman, Jan A. Hobot
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Cell biology., Immunology., Biochemistry., Cell Biology., Immunology., Biochemistry, general.Annat bärarformat
Resin Microscopy and On-Section Immunocytochemistry · ISBN 3-642-47731-3 (Print:)Sammanfattning
Since antibodies tagged with markers have been developed, immunocytochemistry has become the method of choice for identifying tissue substances or for the localisation of nucleic acid in tissue by in situ hybridisation in molecular biology. Resin-embedded tissue is routinely used and new techniques are constantly introduced. Thus, the novice entering these fields has a breathtaking variety of methods open to him. This laboratory book covers the embedding of tissue using less sensitive epoxy resin methods to the more sensitive procedures employing the acrylics. The possibilities are discussed and results are presented so that an understanding of the techniques can be acquired and appropriate choices made. In the first part of the book, the background of the various resins available are discussed and information on inexpensive alternative technologies where they exist is provided. The various steps involved in tissue processing, beginning with fixation, are first described in theory, then detailed protocols are presented for their application, including troubleshooting sections. The second part rationalises the great variety of labelling methods that are commonly used for "on-section" cytochemistry and immunocytochemistry, where colloidal gold is currently overwhelmingly the most popular marker. The principles behind the usage of various markers and their limitations and advantages are discussed.
Innehållsförteckning
I: Resin Embedding -- 1 The Strategic Approach -- 2 The Resins -- 3 Resin Embedding Protocols for Chemically Fixed Tissue -- 4 Cryotechniques -- 5 Methods for Resin Polymerisation -- 6 Handling Resin Blocks -- II: On-Section Immunolabelling -- 7 Strategies in Immunolabelling -- 8 General Considerations -- 9 Immunolabelling Protocols for Resin Sections -- 10 Resin Embedding and Immunolabelling -- Appendix I Examples of Typical Resin Embedding Regimes for Immunocytochemistry -- I.1 Solid Tissue, Pellets and Agar Blocks — Immersion Fixation -- I.2 Solid Tissue — Light Perfusion Fixation -- I.3 Solid Tissue — Very Light Perfusion Fixation -- I.4 Cryosubstitution -- I.5 Project Planner -- Appendix II List of Suppliers -- EM General -- EM Apparatus -- Electron Microscopes -- Flow Cytometry -- References.
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Medverkan och funktion
Geoffrey R. Newman (Författare), Jan A. Hobot (Författare, Författare, http://id.loc.gov/vocabulary/relators/aut)klassifikation
571.6 (DDK-klassifikation)Identifikator
DOI 10.1007/978-3-642-56930-2, ISBN 3642569307Indirekt identifierad av
ISBN 3642477313har titel
Resin Microscopy and On-Section Immunocytochemistryupphovsuppgift
by Geoffrey R. Newman, Jan A. Hobotupplageuppgift
2nd ed. 2001.utgivning
Berlin, Heidelberg : Springer Berlin Heidelberg; Imprint: Springer, 2001omfång
1 online resource (XIX, 273 p.)anmärkning
- Bibliographic Level Mode of Issuance: Monograph
- Includes bibliographical references and index.
Annat bärarformat
Resin Microscopy and On-Section Immunocytochemistry · ISBN 3-642-47731-3 (Print:)Seriemedlemskap
Springer Lab Manuals · Springer Lab Manualskontrollnummer
19202178Resursens ID / Permalänk: https://libris.kb.se/8slxzrbl3thjxr0#it
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