Associations between antinuclear antibody staining patterns and clinical features of systemic lupus erythematosus: analysis of a regional Swedish register

10th International Congress on SLE- Lupus 2013, 18-21 April 2013, Buenos Aires, Argentina, Martina Frodlund (Författare), Örjan Dahlström, 1973- (Författare), Alf Kastbom (Författare), Thomas Skogh (Författare)

URI http://urn.kb.se/resolve?urn=urn:nbn:se:liu:diva-91381, URN urn:nbn:se:liu:diva-91381, DOI 10.1136/bmjopen-2013-003608

Linköpings universitet Institutionen för klinisk och experimentell medicin (Utgivare), Linköpings universitet Hälsouniversitetet (Utgivare), Linköpings universitet Institutionen för beteendevetenskap och lärande (Utgivare), Linköpings universitet Filosofiska fakulteten (Utgivare), Östergötlands Läns Landsting Hjärt- och Medicincentrum (Utgivare)

utgivning

2013

är del av

BMJ Open · ISSN 2044-6055

3, 1-8

Bidrag, Onlineresurs

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http://urn.kb.se/resolve?urn=urn:nbn:se:liu:diva-91381

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ämne

Medical and Health Sciences, Clinical Medicine, Rheumatology and Autoimmunity, Medicin och hälsovetenskap, Klinisk medicin, Reumatologi och inflammation

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Sammanfattning

Objective Antinuclear antibody (ANA) analysis by immunofluorescence (IF) microscopy remains a diagnostic hallmark of systemic lupus erythematosus (SLE). The clinical relevance of ANA fine-specificities in SLE has been addressed repeatedly, whereas studies on IF-ANA staining patterns in relation to disease manifestations are very scarce. This study was performed to elucidate whether different staining patterns associate with distinct SLE phenotypes. Design Observational cohort study. Setting One university hospital rheumatology unit in Sweden. Participants The study population consisted of 222 cases (89% women; 93% Caucasians), where of 178 met ≥4/11 of the 1982 American College of Rheumatology (ACR-82) criteria. The remaining 20% had an SLE diagnosis based on positive IF-ANA (HEp-2 cells) and ≥2 typical organ manifestations at the time of diagnosis (Fries’ criteria). Outcome measures The IF-ANA staining patterns homogenous (H-ANA), speckled (S-ANA), combined homogenous and speckled (HS-ANA), centromeric (C-ANA), nucleolar (N-ANA)±other patterns and other nuclear patterns (oANA) were related to disease manifestations and laboratory measures. Antigen-specificities were also considered regarding double-stranded DNA ( Crithidia luciliae ) and the following extractable nuclear antigens: Ro/SSA, La/SSB, Smith antigen (Sm), small nuclear RNP (snRNP), Scl-70 and Jo-1 (immunodiffusion and/or line-blot technique). Results 54% of the patients with SLE displayed H-ANA, 22% S-ANA, 11% HS-ANA, 9% N-ANA, 1% C-ANA, 2% oANA and 1% were never IF-ANA positive. Staining patterns among patients meeting Fries’ criteria alone did not differ from those fulfilling ACR-82. H-ANA was significantly associated with the 10th criterion according to ACR-82 (‘immunological disorder’). S-ANA was inversely associated with arthritis, ‘immunological disorder’ and signs of organ damage. Conclusions H-ANA is the dominant IF-ANA pattern among Swedish patients with SLE, and was found to associate with ‘immunological disorder’ according to ACR-82. The second most common pattern, S-ANA, associated negatively with arthritis and organ damage.

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URI http://urn.kb.se/resolve?urn=urn:nbn:se:liu:diva-91381, URN urn:nbn:se:liu:diva-91381, DOI 10.1136/bmjopen-2013-003608

har titel

Associations between antinuclear antibody staining patterns and clinical features of systemic lupus erythematosus: analysis of a regional Swedish register