Pre-administration of turmeric prevents methotrexate-induced liver toxicity and oxidative stress [Elektronisk resurs]
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Rezaei Moghadam, Adel (författare)
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Tutunchi, Soheil (författare)
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Namvaran-Abbas-Abad, Ali (författare)
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Yazdi, Mina (författare)
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Bonyadi, Fatemeh (författare)
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Mohajeri, Daryoush (författare)
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Mazani, Mohammad (författare)
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Marzban, Hassan (författare)
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Los, Marek Jan (författare)
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Ghavami, Saeid (författare)
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- Linköpings universitet Institutionen för klinisk och experimentell medicin (utgivare)
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Alternativt namn: IKE
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Alternativt namn: Linköping University. Department of Clinical and Experimental Medicine
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Alternativt namn: Linköping University. Faculty of Health Sciences. Department of Clinical and Experimental Medicine
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Linköpings universitet Medicinska fakulteten (utgivare)
- BioMed Central 2015
- Engelska.
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Ingår i: BMC Complementary and Alternative Medicine. - 1472-6882. ; 15:246
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Sammanfattning
Ämnesord
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- Background: Methotrexate (MTX) is an antimetabolite broadly used in treatment of cancer and autoimmune diseases. MTX-induced hepatotoxicity limits its application. We investigated hepatoprotective effects of turmeric in MTX-induced liver toxicity. Methods: All experiments were performed on male Wistar albino rats that were randomly divided into six groups. Group one received saline orally for 30 days (control group), groups two and three received turmeric extract (100, 200 mg/kg respectively) orally for 30 days, group four received single dose, of MTX IP at day 30, groups five and six received turmeric extract 100 and 200 mg/kg orally respectively for 30 days and single dose of methoterxate IP (20 mg/kg) at day 30. Four days after MTX injection animals were sacrificed and evaluated. Blood ALT and AST (indicators of hepatocyte injury), ALP and bilirubin (markers of biliary function), albumin (reflect liver synthetic function) as well as the plasma TAS concentration (antioxidant defenses) were determined. The cellular antioxidant defense activities were examined in liver tissue samples using SOD, CAT, and GSH-Px for the oxidative stress, and MDA for lipid peroxidation. In addition, liver damage was evaluated histopathologically. Results: MTX significantly induced liver damage (P less than 0.05) and decreased its antioxidant capacity, while turmeric was hepatoprotective. Liver tissue microscopic evaluation showed that MTX treatment induced severe centrilobular and periportal degeneration, hyperemia of portal vein, increased artery inflammatory cells infiltration and necrosis, while all of histopathological changes were attenuated by turmeric (200 mg/kg). Conclusion: Turmeric extract can successfully attenuate MTX-hepatotoxicity. The effect is partly mediated through extracts antinflammatory activity.
Ämnesord
- Medical and Health Sciences (hsv)
- Clinical Medicine (hsv)
- Medicin och hälsovetenskap (hsv)
- Klinisk medicin (hsv)
Indexterm och SAB-rubrik
- Lipid peroxides; Total antioxidant status; Antioxidant enzymes; Serum liver biomarkers; Hepatocellular injury; Cell death
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