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Cellular mechanisms involved in bone resorption [Elektronisk resurs] / by Ulf Lerner

Lerner, Ulf, 1946- (författare)
Umeå universitet. Medicinska fakulteten (utgivare)
Alternativt namn: Umeå universitet. Medicinsk-odontologiska fakulteten
Alternativt namn: Medicinska fakulteten vid Umeå universitet
Umeå : Umeå universitet, 1980
Engelska 68 s.
Serie: Umeå university odontological dissertations. Abstracts, 0345-7532 ; 11
Läs hela texten (Fritt tillgänglig via Umeå universitetsbibliotek)
Läs hela texten (Fritt tillgänglig via Umeå universitetsbibliotek)
  • Bok (digitaliserad)Avhandling(Diss. (sammanfattning) Umeå : Umeå universitet, 1980)
Sammanfattning Ämnesord
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  • The effects of parathyroid hormone (PTH), prostaglandins (PGE 1 , PGE 2 , PGF 2a ), cAMP, cAMP-analogues, phosphodiesterase (PDE) inhibitors and l a (OH) D 3 on bone resorption and associated cellular process have been studied in a bone organ culture system using half- calvaria from 6-7 day-old mice. Bone resorption was assessed by determining the release of calcium (Ca2 2+ ), inorganic phosphate (P i ) and 45 Ca from the calvarial bones to the culture media. The release of lysosomal enzymes was studied by analysing the activities of β-glucuronidase, β-N-acetyl- glucosaminidase, β-galactosidase and p-nitrophenyl phosphatase in bone expiants and culture media. The release of non-lysosomal enzymes was followed by assaying the activities of lactate dehydrogenase (LDH), aspartate aminotransferase (ASAT) and alanine aminotransferase (ALAT) in the expiants as well as the media. In addition glucose consumption and lactate production was registered. The findings may be summarized as follows:1. cAMP and PDE-inhibitors have the capacity to inhibit the initial stages of spontaneous as well as PTH- PGE 1 - and PGE 2 -stimulated bone resorption. 2. cAMP and PDE-inhibitors produce after a lag period, or a period of reduced bone resorption, a stimulatory effect on bone resorption. 3. There is a significant correlation between bone resorption and lysosomal enzyme release both as regards the inhibitory and stimulatory effect of cAMP. 4. PGE 2 and l a (OH) D 3 increase the release of lysosomal enzymes in parallel with bone resorption. 5. Bone resorption stimulated by cAMP and PGE 2 is associated with increased glucose consumption and lactate production, while l a (OH) D 3 promotes bone resorption without any change with regard to these parameters of glucose metabolism. It is concluded that the initial stages of bone resorption stimulated by PTH, PGE 1 and PGE 2 is medited by cAMP-independent mechanisms, but that this nucleotide may be an intracellular mediator of these hormones of later stages of bone resorption. It is suggested that the role played by cAMP may be related to the capacity of PTH and PGE2 to develop new osteoclasts. The observations further support the concept that lysosomal enzyme release is intimately associated with bone resorption. Finally it is concluded that increased lactate production seems to be related to bone resorption stimulated by agents which increase the level of cAMP (PTH, PGE 2 , dbcAMP), but that it is not an indispensible part of the mechanism by which the osteoclasts solubilize bone mineral. 

Ämnesord

Bone resorption  (MeSH)
Medical and Health Sciences  (hsv)
Medicin och hälsovetenskap  (hsv)

Indexterm och SAB-rubrik

Bone resorption. cyclic AMP
parathyroid hormone
prostaglandins
la (OH) D3
lysosomal enzyme release
osteoclast
periodontitis
rheumatoid arthritis

Klassifikation

617.6 (DDC)
Vk (kssb)
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